Mission Bio Technology

Problem

The analysis of single cells within complex biological systems can reveal critical information not observable from bulk sample analysis. Emerging technologies for single-cell molecular analysis have resolution down to individual cells, but can only analyze at most hundreds of cells. These approaches are not scalable to the throughput levels required by researchers and clinicians studying rare cells and cellular heterogeneity.

Solution

Mission Bio provides tools for the study of cellular heterogeneity in human health and life science research. Our approach relies on proprietary microfluidic droplet technology to deliver rapid and cost-effective solutions for molecular analysis of single cells.  Please explore our publications for additional information on the science behind Mission Bio’s technology.

Publications

RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures

M. Pellegrino, A. Sciambi, J.L. Yates, J. Mast, C. Silver, D.J. Eastburn,  BMC Genomics. (2016).

Ultrahigh-Throughput Mammalian Single-Cell Reverse-Transcriptase Polymerase Chain Reaction in Microfluidic Drops

D.J. Eastburn, A. Sciambi, A.R. Abate, Analytical Chemistry. 85, 8016 (2013).

Microfluidic droplet enrichment for targeted sequencing

D.J. Eastburn, Y. Huang, M. Pellegrino, A. Sciambi, L. Ptáček, A. Abate, Nucleic Acids Research. (2015).

Picoinjection enables digital detection of RNA with droplet rt-PCR

D.J. Eastburn, A. Sciambi, A.R. Abate, PLoS ONE. 8(4): e62961 (2013).

Identification and genetic analysis of cancer cells with PCR-activated cell sorting

D.J. Eastburn, A. Sciambi, A.R. Abate, Nucleic Acids Research. 42, e128 (2014).