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publication

RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.


Pellegrino, M. et al.
BMC Genomics (2016)
Abstract

Rare cell subtypes can profoundly impact the course of human health and disease, yet their presence within a sample is often missed with bulk molecular analysis. Single-cell analysis tools such as FACS, FISH-FC and single-cell barcode-based sequencing can investigate cellular heterogeneity; however, they have significant limitations that impede their ability to identify and transcriptionally characterize many rare cell subpopulations.



Authors

Pellegrino, M., Sciambi, A., Yates, J.L., Mast, J.D., Silver, C., Eastburn, D.J.



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