Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia (AEL) CRISPR Induced Mouse Model

Acute erythroid leukemia (AEL) is a high-risk form of acute myeloid leukemia, but models of this rare disease are lacking. In this application note, CRISPR and single-cell DNA sequencing are used to generate and analyze mouse models of AEL. Single-cell analysis of serially transplanted tumors revealed the clonal structure and co-mutations responsible for tumorigenesis.

The following are covered:

• Assessment of precise genetic edits
• Analysis of the clonal structure & co-mutations of AEL
• Use of CRISPR and scDNA-seq for disease modeling