Why Single Cell for Hematologic Malignancy Applications?
For hematologic malignancies, simultaneous DNA and protein analysis from the same cell can resolve the zygosity of mutations, determine if mutations co-occur or are mutually exclusive, and correlate that to immunophenotypes. With these insights, phylogenetic trees can be reconstructed to determine the order of acquisition of these mutations. Fish plots show how clonal architecture changes throughout the course of treatment. And monitoring during remission poses great promise for sensitive MRD detection.
It is time to get a holistic view of cancer
Track clonal evolution and reconstruct phylogenetic trees
Clonal evolution of acute myeloid leukemia revealed by high-throughput single-cell genomics
Dr. Koichi Takahashi, MD Anderson Cancer Center
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Reveal therapy resistance mechanisms through course of treatment
Clonal selection with Ras pathway activation mediates secondary clinical resistance to selective FLT3 inhibition in AML
Dr. Cathy Smith, Dr. Alexander Perl, UCSF, UPenn
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Monitor disease during remission to track MRD
Single-cell mutational profiling enhances the clinical evaluation of AML MRD
Dr. Ravi Majeti, Stanford
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