Clonal Evolution of Acute Myeloid Leukemia revealed by high-throughput single-cell genomics
Morita K, Wang F, Jahn K, Hu T, Tanaka T, Sasaki Y, Kuipers J, Loghavi S, Wang SA, Yan Y, Furudate K, Matthews J, Little L, Gumbs C, Zhang J, Song X, Thompson E, Patel KP, Bueso-Ramos CE, DiNardo CD, Ravandi F, Jabbour E, Andreeff M, Cortes J, Bhalla K, Garcia-Manero G, Kantarjian H, Konopleva M, Nakada D, Navin N, Beerenwinkel N, Futreal PA, Takahashi K.
Nature Communications
Oct 2020
Abstract
Clonal diversity is a consequence of cancer cell evolution driven by Darwinian selection. Precise characterization of clonal architecture is essential to understand the evolutionary history of tumor development and its association with treatment resistance. Here, using a single-cell DNA sequencing, we report the clonal architecture and mutational histories of 123 acute myeloid leukemia (AML) patients. The single-cell data reveals cell-level mutation co-occurrence and enables reconstruction of mutational histories characterized by linear and branching patterns of clonal evolution, with the latter including convergent evolution. Through xenotransplantion, we show leukemia initiating capabilities of individual subclones evolving in parallel. Also, by simultaneous single-cell DNA and cell surface protein analysis, we illustrate both genetic and phenotypic evolution in AML. Lastly, single-cell analysis of longitudinal samples reveals underlying evolutionary process of therapeutic resistance. Together, these data unravel clonal diversity and evolution patterns of AML, and highlight their clinical relevance in the era of precision medicine.
VIEW
Area
Heme
Institution Type
Academia
Indication / Modality
Acute Myleoid Leukemia (AML)
Goal of Study
MRD, Therapeutic Resistance, Retrospective, Disease Progression, Disease Modeling, Longitudinal
Key Genes
NPM1, FLT3, DNMT3A, RAS, IDH2, RUNX1, TET2
PAD Project
No
Analytes Assessed
SNV, InDels
Sample Storage
Fresh Frozen
Sample Prep
Whole Cells
Sample Type
Patient Material
Tissue / Organ
Bone Marrow Aspirates
Species
Human
Panel Used
Catalog AML Panel
Proof Point Demonstrated
Clonality, Survival Curves, Co-occurrence, Zygosity, Better than Bulk, Sensitivity, Multi-omics, Phylogeny
