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Scientific presentation

Clonal Heterogeneity in Acute Lymphoblastic Leukemia at Diagnosis and During Chemo Treatment


Jan Cools, PhD, VIB Center for Cancer Biology
Description

Acute lymphoblastic leukemia (ALL) is aggressive leukemia that occurs most frequently in children and is characterized by the presence of few chromosomal rearrangements and additional mutations. In this webinar, the second in a “Meet the Authors” series, Jan Cools of the VIB Center for Cancer Biology in Leuven, Belgium will discuss the application of single-cell analysis to determine the clonal heterogeneity of the leukemia cells of ALL cases at diagnosis.

Dr. Cools’ lab used single-cell targeted DNA sequencing (Tapestri, Mission Bio) and single-cell RNA-sequencing (10x Genomics) to determine the clonal heterogeneity of the leukemia cells of 20 ALL cases at diagnosis and monitored the clonal evolution during chemotherapy treatment. Specifically, the lab designed a custom ALL panel and obtained accurate single-nucleotide variant and small insertion-deletion mutation calling for 305 amplicons covering 110 genes in about 4400 cells per sample and time point. Bone marrow and/or blood samples from 12 B-cell ALL and eight T-cell ALL patients were analyzed.

Dr. Cools will discuss how single-cell DNA amplicon sequencing is a sensitive assay to detect clonal architecture and evolution of the malignant cells in ALL, and present his findings published in the Blood paper, “Single-cell DNA amplicon sequencing reveals clonal heterogeneity and evolution in T-cell acute lymphoblastic leukemia.”



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