Sample dataset for a 4-cell line mixed experiment using the Tapestri Single-Cell DNA AML_v2 panel

 

  • A cell line mix consisting of 43% Mutz-8, 26% Raji, 20% K562 and 11% Jurkat cells was run on the Tapestri Platform
  • 1/4 of the Tapestri run was sequenced on an Illumina NextSeq instrument
  • FASTQ files were processed with Tapestri Pipeline software using default values for parameters to generate variant calls
  • 79M reads were generated for 1,241 cells at ~3,859 reads per cell and 30x coverage of reads/amplicon/cell
  • Download includes amplicon.bed for AML_v2 panel, barcode distribution file, VCF header file & LOOM file, which can be used as input to the tapestri.cnv R package