Single-cell Multiomics Powers Biomarker Insights into AML

The heterogeneity and dynamism of AML clonal architecture pose a challenge for MRD monitoring. Yet, MRD assessment is crucial for prognosis, as MRD positivity post-remission indicates a higher relapse risk. Conventional single-analyte methods like flow cytometry and PCR are unable to detect the immunophenotypic or genotypic changes arising during treatment. While error-corrected NGS offers highly sensitive detection of residual cells and simultaneous evaluation of many genes, it cannot differentiate true AML from precursor clones, leading to false-positive results. Even when combining these assays to achieve clinical utility, integrating data from different modalities remains challenging.