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Publications

scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells


Bianchi A, Scherer M, Zaurin R, Quililan K, Velten L, Beekman R.
Genome Biology Oct 2022
Abstract

Single-cell DNA methylation profiling currently suffers from excessive noise and/or limited cellular throughput. We developed scTAM-seq, a targeted bisulfite-free method for profiling up to 650 CpGs in up to 10,000 cells per experiment, with a dropout rate as low as 7%. We demonstrate that scTAM-seq can resolve DNA methylation dynamics across B-cell differentiation in blood and bone marrow, identifying intermediate differentiation states that were previously masked. scTAM-seq additionally queries surface-protein expression, thus enabling integration of single-cell DNA methylation information with cell atlas data. In summary, scTAM-seq is a high-throughput, high-confidence method for analyzing DNA methylation at single-CpG resolution across thousands of single cells.

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Area

Heme

Institution Type

Academia

Indication / Modality

Other

Goal of Study

DNA Methylation

PAD Project

No

Analytes Assessed

SNV, Extracellular Protein, DNA Methylation

Sample Storage

Fresh Frozen

Sample Prep

Whole Cells

Sample Type

PBMC

Tissue / Organ

Bone Marrow Aspirates

Species

Human

Panel Used

Custom

Proof Point Demonstrated

Better than Bulk, Cell Identity, Multi-omics
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