Most meningiomas, a type of tumor that arise from the meningeal layers of the brain and spinal cord, carry mutations in the neurofibromatosis gene 2 (NF2) while NF2-wildtype meningiomas account for 1/3rd of all meningiomas. NF2-wild type meningiomas are commonly associated with mutations in SMO, POLR2A, PIK3CA, AKT1, and KLF4 mutations, the latter both occur regularly with TRAF7 mutations. The order of mutational acquisition, whether alterations in TRAF7 or in AKT1/KLF4 occurs first, remains elusive. Here we see that single-cell DNA sequencing helped elucidate the order of mutational acquisition in TRAF7/AKT1 and TRAF7/KLF4 mutant meningiomas which bulk sequencing failed to resolve.
Helin Dogan
