Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
X
X
publication

High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics.


Pellegrino, M. et al.
Genome Research (2018)
Abstract

To enable the characterization of genetic heterogeneity in tumor cell populations, we developed a novel microfluidic approach that barcodes amplified genomic DNA from thousands of individual cancer cells confined to droplets. The barcodes are then used to reassemble the genetic profiles of cells from next-generation sequencing data. By using this approach, we sequenced longitudinally collected acute myeloid leukemia (AML) tumor populations from two patients and genotyped up to 62 disease relevant loci across more than 16,000 individual cells. Targeted single-cell sequencing was able to sensitively identify cells harboring pathogenic mutations during complete remission and uncovered complex clonal evolution within AML tumors that was not observable with bulk sequencing. We anticipate that this approach will make feasible the routine analysis of AML heterogeneity, leading to improved stratification and therapy selection for the disease.



Authors

Pellegrino, M., Sciambi, A., Treusch, S., Durruthy-Durruthy, R., Gokhale, K., Jacob, J., Chen, T.X., Geis, J.A., Oldham, W., Matthews, J., Kantarjian, H., Futreal, P.A., Patel, K., Jones, K.W., Takahashi, K., Eastburn, D.J.



VIEW

publication
A JAK2/IDH1-mutant MPN clone unmasked by ivosidenib in an AML patient without antecedent MPN.
Xiao, Wenbin
Blood Advances (2020)
publication
Single-cell mutation analysis of clonal evolution in myeloid malignancies.
Miles, L. A.
Nature (2020)
publication
Leukemia stemness and co-occurring mutations drive resistance to IDH inhibitors in acute myeloid leukemia
Wang, F.
BioRxiv (2020)
publication
Personalized Single-Cell Proteogenomics to Distinguish Acute Myeloid Leukemia from Non-Malignant Clonal Hematopoiesis
Laura W. Dillon
MedRxiv (2020)
REQUEST QUOTE