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Announcing new Tapestri Platform updates including lowered input threshold and expanded heme-onc menu. Learn More
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blog
September 13, 2021 by Brittany Enzmann, PhD 3 min read

Introducing Mission Bio’s Published Panels!

Mission Bio is very excited to launch our New Tapestri Single-cell DNA Published Panels! We are expanding our heme-oncology menu with pre-designed, ready-to-ship panels sourced from researchers and featured in peer-reviewed publications. 

Published Panel Highlights:

  • Demonstrated performance on clinical samples via publications in high impact peer-reviewed journals 
  • Pre-designed, ready-to-use content accelerates oligo designing & ordering (content curated by investigators in each disease indication)
  • Available in a smaller 8-sample pack size compared to custom panels, providing cost-efficiency for pilot projects

Our Published Panels are now easily orderable here

1)  Acute Lymphoblastic Leukemia Published Panel 

The Tapestri Single-cell DNA Acute Lymphoblastic Leukemia (ALL) Published Panel was designed by the Jan Cools Lab at the Center for Cancer Biology, Vlaams Instituut voor Biotechnologie (VIB). This panel targets 112 genes with 305 amplicons for single-cell sequencing, designed to target the most commonly mutated hotspots in ALL, as defined by COSMIC and PeCan databases.

The ALL Published Panel is featured in a study published in Blood, “Single-cell DNA amplicon sequencing reveals clonal heterogeneity and evolution in T-cell acute lymphoblastic leukemia.

  • Researchers assessed ALL variant hotspots across 305 genomic regions by analyzing 108,188 cells for 25 samples of 8 T-ALL patients to explore the clonality underlying T-ALL as well as the use for single-cell sequencing for MRD detection. In their study, the researchers found that T-ALL samples were polyclonal, with NOTCH1 mutations commonly observed, and minor clones at diagnosis sometimes became major clones at later disease stages.

2) Myeloproliferative Neoplasms Published Panel 

The Tapestri Single-cell DNA Myeloproliferative Neoplasms (MPN) Published Panel was designed by the Piers Blombery Lab at Peter MacCallum Cancer Center. This panel targets 18 genes with 70 amplicons for single-cell sequencing, designed to target the most commonly mutated hotspots involved in MPN.

The MPN Published Panel is featured in a study published in Haematologica, “Clonal independence of JAK2 and CALR or MPL mutations in comutated myeloproliferative neoplasms demonstrated by single cell DNA sequencing.

  • Researchers investigated the clonal relationship of the JAK2 and CALR mutations and of the JAK2 and MPL mutations in MPN patients using single-cell mutation analysis of mononuclear cells to explore potential mutation co-occurrence events and mutually exclusive mutations in two patients that each harbor two driver mutations. In their study, the researchers found that scDNA-seq confirmed that the driver mutations were in different clones and the assessment of these canonical mutations was necessary to characterize disease biology.

3) Myeloid Clonal Evolution Published Panel 

Tapestri Single-cell DNA Myeloid Clonal Evolution Published Panel was designed by the Ross Levine Lab at Memorial Sloan Kettering Cancer Center. This panel targets 32 genes with 109 amplicons for single-cell sequencing, designed to target the most commonly mutated hotspots found to be frequently mutated in human myelodysplastic syndromes (MDS), myeloproliferative neoplasms (MPN), and acute myeloid leukemia (AML).

The Myeloid Clonal Evolution Published Panel was featured in a study published in Nature, “Single-cell mutation analysis of clonal evolution in myeloid malignancies.”

  • The team examined the order of mutation acquisition, impact of co-occurring mutations on clonal fitness and expansion, and correlation between co-mutations and immunophenotypic profiles across clonal hematopoiesis (CH), myeloproliferative neoplasms (MPN) and acute myeloid leukemia (AML) by sequencing 740,529 cells from 146 samples from 123 patients with myeloid malignancies. In the study, the researchers used scDNA-seq to reveal disease-specific clonal architecture and evolution, and they used single-cell multi-omics to uncover genotype-driven changes to immunophenotypes.

Mission Bio is very excited to launch our new Published Panels to help you kickstart your projects! Get started today!

Register for our webinar on September 21st  featuring Dr. Koichi Takahashi of MD Anderson Cancer Center to learn about the applications of single-cell analysis in myeloid malignancies. 

 

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