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September 9, 2021 by James Flynn, PhD 2 min read

Low Input, Big Insights

Single-cell analysis has enabled the comprehensive characterization of tumor samples, especially for blood cancers like leukemia. Using the Tapestri Platform, for instance, enables researchers to dissect the clonal structure of complex samples and discover the genotypic signatures of individual cells. 

One challenge any scientist will encounter, however, is the desire to run many experiments with a limited amount of precious sample material. This fundamental issue can be a barrier to applying single-cell methods in studies where the sample requirements are simply too high.  

Mission Bio has heard this call and now has validated the Tapestri Platform for low cell inputs, down to as few as 20,000 cells. This low cell input requirement will allow you to use your precious samples while still maintaining excellent scDNA-seq sensitivity to accurately call single nucleotide variants (SNVs). This newly validated feature is a 5x reduction in the original cell input requirement, providing access to smaller samples and enabling new applications which previously could not be processed using the Tapestri Platform due to small numbers of cells available.

Low cell input is well suited to applications under development at Mission Bio such as small solid tumor samples or limited cell biopsy from model organisms such as PDX mouse models or organoid cell cultures.  The ability to utilize low cell inputs is now available with every Tapestri device on market and provides an excellent foundation for the expansion of the existing applications performed on our single-cell DNA-seq platform.  


Low cell input (20K cells).                                                                                                                  High cell input (100K cells)

Heat map for Tapestri 20k cell inputHeat map for Tapestri 100k cell input

Consistent variant signatures and sensitivity were observed in low (20k, left) and high (100k, right) inputs of three cell lines. The cell line GM12878 (spiked-in at 5% concentration, in red ) is detected consistently, demonstrating that the ability to detect cell populations is unaffected by cell input amounts.

Learn more about the low cell input requirement on the Tapestri Platform here.

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We are always here to chat. Please reach out to one of our team to learn more about how single-cell genomics can apply to your research at unprecedented resolution.   

About the Author

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