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Announcing the new Tapestri scMRD Assay for AML. Learn More
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poster

Precise Measurement of Viral Copy Number at Single-Cell Resolution for Cell and Gene Therapy Development


Yue Wang

Cell and gene therapies are transformative solutions for a host of inherited and acquired diseases for which existing interventions are ineffective. Many such therapies rely on the introduction of transgenes into host cells using viral or non-viral vectors. The accurate measurement of gene transfer is critical to the development of therapeutic agents and is a key attribute for assessing their safety and efficacy. Yet, conventional methods for measuring gene transfer lack the resolution and representation to truly reflect sample composition and either report a population average (bulk) or involve laborious and time-consuming clonal outgrowth which can take weeks. Mission Bio has developed an end-to-end solution from panel design to data analysis for single-cell targeted DNA sequencing. Here, using the Tapestri platform we demonstrate that single-cell DNA sequencing identifies transduced versus non-transduced cells with exceptional accuracy and precision, as well as measures the single-cell level vector copy number (VCN) for populations of thousands of cells with single nucleotide resolution while reducing sample processing time from weeks to days.


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poster
Leveraging Single-cell DNA Sequencing for In-depth Characterization of Cell and Gene Therapies
Jacqueline Marin, Benjamin Schroeder, Shu Wang, Daniel Mendoza, Adam Sciambi, Brittany Enzmann
poster
Multimodal Analysis of DNA and Proteins in Single Cells
Prithvi Singh, Dalia Dhingra, Saurabh Parikh, Adam Sciambi, Aik Ooi
poster
Precise Measurement of Transduction Efficiency at Single-Cell Resolution for Cell and Gene Therapy Development
Khushali Patel
poster
Enabling single cell analysis of copy number variation in breast cancer
Jacqueline Marin
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