Evolving subclonal mutations can resist and escape treatment

"We have a profound gap in knowledge between the interaction of a person, their cancer, and their therapy and how that impacts response. We need better insight." — Jerry Radich, MD

Break Out of the Limitations of Bulk.

The average read-out from conventional bulk sequencing misses the underlying genetic diversity across cell populations. To improve patient stratification, therapy selection, and disease monitoring we need insights into mutation co-occurrence within every single cell.

Tapestri™

The industry’s first scalable, customizable, single-cell DNA analysis platform.

The Mission Bio Tapestri Platform was developed to help advance precision medicine by enabling the accelerated and accessible detection of genomic variability within and across cell populations. Leveraging proprietary droplet microfluidics, the platform unlocks access to DNA at the single-cell level with a novel two-step protease workflow. This methodology provides flexibility for additional applications and customization capability.

How the Tapestri Platform Works

Step 1: Tapestri Instrument partitions cells into droplets along with a protease enzyme mix. Cell lysis and protease digestion then follow off-instrument enabling access to DNA for downstream amplification.

Step 2: The cell lysate is then combined with barcoded beads, primers, and reagents. Specific regions of interest are then amplified while tagging each amplicon with a unique cell barcode, preserving the cell’s identity and its mutational profile.

Step 3: After running your sequencer, proceed with our downstream analysis and visualization software. Mission Bio provides dedicated bioinformatics support to help you discover biological and clinical insights

High Sensitivity to Reveal True Heterogeneity

The Tapestri Platform revolutionizes the capability to directly assess the clonal architecture of a sample with detection of mutation co-occurrence patterns. Rather than inferring variants that co-occur within a subclone from comparable bulk variant allele frequencies, single-cell resolution uncovers the true distribution of genotypes and their segregation patterns across subclones.

  • Detect rare subclones down to 0.1%
  • Resolve clonal architecture
  • Identify mutation co-occurrence

Platform Features

  • Targeted and accurate SNVs and indel variant calling
  • Single-cell DNA throughput up to 10,000 cells
  • Simple workflow
  • User-friendly bioinformatics software
  • Customizable content